Single-Step Process for Genomic Target Capture
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Description:
The Naval Research Laboratory has developed a magnetic microbead support that enables the capture of genomic targets in a single step. The microbead is functionalized with a phosphorous branched dendrimer linker, which covalently binds DNA capture probes, allowing hybridization of the target directly onto the bead. The targets can then be recovered through heat denaturation in small elution volumes for direct analysis. The robust bond between capture probe and microbead permits the regeneration of the probe even after repeated cycles at high temperatures (up to 100°C) and salt conditions (3-5 molar NaCl), providing a reusable universal platform for sequence-capture or background subtraction. Use of variable branched dendrimeric linker systems enables the performance to be tailored for specific targets.Advantages/Features Include:
- Time-saving single-step process leads to increased profits
- Reusable probe allows for rapid regeneration with smaller elution volumes for greater sample throughput
- Dendrimeric linker provides a larger spacing between the probe and the surface reducing steric hindrance and enhancing the capture of longer targets
Applications Include:
- Clinical diagnostics
- Environmental surveillance of biological agents
- Genomic profiling
- Portable sensor devices for first responders
References:
- "Magnetic Bead-Based Solid Phase for Selective Extraction of Genomic DNA," Analytical Biochemistry, 355 (2006) 285-297.
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Contact:
Naval Research LaboratoryTechnology Transfer Office, Code 1004
techtran@research.nrl.navy.mil