The BioEnergy & BioFabrication section has a wide range of biological expertise with a focus in energy and biofabrication applications. Bioenergy capabilities range from basic investigations into microbial metabolism and extracellular electron transfer mechanisms to more applied application of biological systems for energy production. Biofabrication efforts are focused on creating heterogeneous three dimensional in vitro tissues via biological laser printing (BioLP) and the use of BioLP to isolate novel microorganisms from environmental samples.

In addition the section has a variety of bioanalytical capabilities including culture facilities (microorganisms and mammalian cells), analytical techniques (thermogravimetric analysis, GC, HPLC, and mass spectrometry (LC/MS/MSn)) for analysis of proteins, fuels or metabolites, molecular biology techniques (polymerase chain reaction (PCR), gel electrophoresis, and real time PCR).

Selected Publications

2017 Cusick, K.D.., J.R.. Dale, L.A.. Fitzgerald, B.J.. Little, and J.C.. Biffinger, "Adaptation to Copper Stress Influences Biofilm Formation in Alteromonas Macleodii", Biofouling, vol. 33, 2017.
2017 "Adaptation to copper stress influences biofilm formation in Alteromonas macleodii", Biofouling, vol. 33, issue 6, 2017.
2017 "Gene Expression Changes in Long-Term in Vitro Human Blood-Brain Barrier Models and their Dependence on a Transwell Scaffold Material", Journal of Healthcare Engineering, issue 5740975, 2017.
2017 "Spatial variation in sediment organic carbon distribution across the Alaskan beaufort sea shelf", Energies, vol. 10, issue 9, 2017.
2016 "Molecular mechanisms contributing to the growth and physiology of an extremophile cultured with dielectric heating", Applied and Environmental Microbiology, vol. 82, issue 20, 2016.
2016 "Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood-brain barrier tissue", Journal of Biomedical Materials Research – Part A, vol. 104, issue 4, 2016.
2016 "Aerosol meteorology of Maritime Continent for the 2012 7SEAS southwest monsoon intensive study-Part 2: Philippine receptor observations of fine-scale aerosol behavior", Atmospheric Chemistry and Physics, vol. 16, issue 22, 2016.
2016 "Measuring carbon-based contaminant mineralization using combined Co2 flux and radiocarbon analyses", Journal of Visualized Experiments, issue 116, 2016.
2016 "Optical proxies for terrestrial dissolved organic matter in estuaries and coastal waters", Frontiers in Marine Science, vol. 2, issue Jan, 2016.
2015 "Development of a compartmentalized biochip for axonal isolation and neuronal-circuit formation at the single-cell level", Neuromethods, vol. 103, issue 83-104, 2015.
2015 "Combined radiocarbon and CO2 flux measurements used to determine in situ chlorinated solvent mineralization rate", Environmental Sciences: Processes and Impacts, vol. 17, issue 3, 2015.
2015 "Observations of the temporal variability in aerosol properties and their relationships to meteorology in the summer monsoonal South China Sea/East Sea", Atmospheric Chemistry and Physics, vol. 15, issue 4, 2015.
2015 "The applicability of Impranil® DLN for gauging the biodegradation of polyurethanes", Polymer Degradation and Stability, vol. 120, issue 7688, 2015.
2015 "Selection and evaluation of reference genes for reverse transcription-quantitative PCR expression studies in a thermophilic bacterium grown under different culture conditions", PLoS ONE, vol. 10, issue 6, 2015.
2015 "Suppressing the Neurospora crassa circadian clock while maintaining light responsiveness in continuous stirred tank reactors", Scientific Reports, vol. 5, 2015.
2015 "Printing soil: A single-step, high-throughput method to isolate micro-organisms and near-neighbour microbial consortia from a complex environmental sample", Methods in Ecology and Evolution, vol. 6, issue 2, 2015.
2015 "Differences in physical and biochemical properties of Thermus scotoductus SA-01 cultured with dielectric or convection heating", Applied and Environmental Microbiology, vol. 81, issue 18, 2015.
2015 "Development of a compartmentalized biochip for axonal isolation and neuronal-circuit formation at the single-cell level (Book Chapter)", Microfluidic and Compartmentalized Platforms for Neurobiological Research, 2015.
2014 "Selection and evaluation of reference genes for expression studies with quantitative PCR in the model fungus Neurospora crassa under different environmental conditions in continuous culture", PLoS ONE, vol. 9, issue 12, 2014.
2014 "Nanoparticle facilitated extracellular electron transfer in microbial fuel cells", Nano Letters, vol. 14, issue 11, 2014.
2014 "A direct quantitative agar-plate based assay for analysis of Pseudomonas protegens Pf-5 degradation of polyurethane films", International Biodeterioration and Biodegradation, vol. 95, issue PB, 2014.
2014 "Computer aided design and manufacturing of soft, three-dimensional, multilayer, biological constructs via laser printing onto laser machined composite biopapers", Journal of Imaging Science and Technology, vol. 58, issue 4, 2014.
2013 "Probing single- to multi-cell level charge transport in Geobacter sulfurreducens DL-1", Nature Communications, vol. 4, 2013.
2013 "Shewanella frigidimarina microbial fuel cells and the influence of divalent cations on current output", Biosensors and Bioelectronics, vol. 40, issue 1, 2013.
2013 "Controlling autonomous underwater floating platforms using bacterial fermentation", Applied Microbiology and Biotechnology, vol. 97, issue 1, 2013.
2013 "Cell and organ printing turns 15: Diverse research to commercial transitions", MRS Bulletin, vol. 38, issue 10, 2013.
2013 "Radiocarbon-depleted CO2 evidence for fuel biodegradation at the Naval Air Station North Island (USA) fuel farm site", Environmental Sciences: Processes and Impacts, vol. 15, issue 5, 2013.
2013 "Incorporation and mineralization of TNT and other anthropogenic organics by natural microbial assemblages from a small, tropical estuary", Environmental Pollution, vol. 174, 2013.